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anti-αe blocking antibody clone number m290  (Bio X Cell)


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    Bio X Cell anti-αe blocking antibody clone number m290
    αEβ7 <t>or</t> <t>E-cadherin</t> blockade inhibits T cell interactions with intestinal epithelium Adoptive transfer of tdTomato + OT-1 cells into WT or E-cadherin-CFP recipients was followed by oral challenge with cholera toxin:OVA the following day. Three days after immunization, recipient mice were treated with isotype or <t>anti-αE</t> or anti-E-cadherin antibodies before analysis. (A) OT-1 cell number in the small intestine lamina propria. Bar graph shows means ± SEM of six to eight mice combined from two independent experiments. (B) Confocal microscopy image of intestinal tissue sections stained with anti-E-cadherin (green) and anti-laminin (blue). White arrows point to tdTomato (red) OT-1 T cells in close proximity to E-cadherin on basolateral epithelium. Scale bar: 100 μm. Data are representative of three independent experiments. (C) Representative movie of tdTomato (red) OT-1 T cells interacting with intestinal epithelium (green) in control or anti-αE-treated mice. The basement membrane is indicated by a dashed white line. Scale bar: 20 μm. (D and E) Maximal track speed (D) and track length (E) were quantified for individual cells. Bar graph shows means ± SEM, and each dot represents an individual cell migration event. Data are representative of five independent experiments. One-way ANOVA with Dunnett’s post-test or unpaired Student‘s t test was used to calculate statistical significance. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.
    Anti αe Blocking Antibody Clone Number M290, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti-%CE%B1e+blocking+antibody+clone+number+m290/anti+cd103+m290/pmc08385326-341-0-8
    Average 90 stars, based on 1 article reviews
    anti-αe blocking antibody clone number m290 - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Dual targeting of lymphocyte homing and retention through α4β7 and αEβ7 inhibition in inflammatory bowel disease"

    Article Title: Dual targeting of lymphocyte homing and retention through α4β7 and αEβ7 inhibition in inflammatory bowel disease

    Journal: Cell Reports Medicine

    doi: 10.1016/j.xcrm.2021.100381

    αEβ7 or E-cadherin blockade inhibits T cell interactions with intestinal epithelium Adoptive transfer of tdTomato + OT-1 cells into WT or E-cadherin-CFP recipients was followed by oral challenge with cholera toxin:OVA the following day. Three days after immunization, recipient mice were treated with isotype or anti-αE or anti-E-cadherin antibodies before analysis. (A) OT-1 cell number in the small intestine lamina propria. Bar graph shows means ± SEM of six to eight mice combined from two independent experiments. (B) Confocal microscopy image of intestinal tissue sections stained with anti-E-cadherin (green) and anti-laminin (blue). White arrows point to tdTomato (red) OT-1 T cells in close proximity to E-cadherin on basolateral epithelium. Scale bar: 100 μm. Data are representative of three independent experiments. (C) Representative movie of tdTomato (red) OT-1 T cells interacting with intestinal epithelium (green) in control or anti-αE-treated mice. The basement membrane is indicated by a dashed white line. Scale bar: 20 μm. (D and E) Maximal track speed (D) and track length (E) were quantified for individual cells. Bar graph shows means ± SEM, and each dot represents an individual cell migration event. Data are representative of five independent experiments. One-way ANOVA with Dunnett’s post-test or unpaired Student‘s t test was used to calculate statistical significance. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.
    Figure Legend Snippet: αEβ7 or E-cadherin blockade inhibits T cell interactions with intestinal epithelium Adoptive transfer of tdTomato + OT-1 cells into WT or E-cadherin-CFP recipients was followed by oral challenge with cholera toxin:OVA the following day. Three days after immunization, recipient mice were treated with isotype or anti-αE or anti-E-cadherin antibodies before analysis. (A) OT-1 cell number in the small intestine lamina propria. Bar graph shows means ± SEM of six to eight mice combined from two independent experiments. (B) Confocal microscopy image of intestinal tissue sections stained with anti-E-cadherin (green) and anti-laminin (blue). White arrows point to tdTomato (red) OT-1 T cells in close proximity to E-cadherin on basolateral epithelium. Scale bar: 100 μm. Data are representative of three independent experiments. (C) Representative movie of tdTomato (red) OT-1 T cells interacting with intestinal epithelium (green) in control or anti-αE-treated mice. The basement membrane is indicated by a dashed white line. Scale bar: 20 μm. (D and E) Maximal track speed (D) and track length (E) were quantified for individual cells. Bar graph shows means ± SEM, and each dot represents an individual cell migration event. Data are representative of five independent experiments. One-way ANOVA with Dunnett’s post-test or unpaired Student‘s t test was used to calculate statistical significance. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

    Techniques Used: Adoptive Transfer Assay, Confocal Microscopy, Staining, Control, Membrane, Migration

    Related Articles

    Blocking Assay:

    Article Title: Dual targeting of lymphocyte homing and retention through α4β7 and αEβ7 inhibition in inflammatory bowel disease
    Article Snippet: Anti-αE blocking antibody (clone number M290) was from BioXcell and anti-E-cadherin antibody (clone number ECCD-2) was from Invitrogen.

    Adoptive Transfer Assay:

    Article Title: Dual targeting of lymphocyte homing and retention through α4β7 and αEβ7 inhibition in inflammatory bowel disease
    Article Snippet: Anti-αE blocking antibody (clone number M290) was from BioXcell and anti-E-cadherin antibody (clone number ECCD-2) was from Invitrogen.

    Confocal Microscopy:

    Article Title: Dual targeting of lymphocyte homing and retention through α4β7 and αEβ7 inhibition in inflammatory bowel disease
    Article Snippet: Anti-αE blocking antibody (clone number M290) was from BioXcell and anti-E-cadherin antibody (clone number ECCD-2) was from Invitrogen.

    Staining:

    Article Title: Dual targeting of lymphocyte homing and retention through α4β7 and αEβ7 inhibition in inflammatory bowel disease
    Article Snippet: Anti-αE blocking antibody (clone number M290) was from BioXcell and anti-E-cadherin antibody (clone number ECCD-2) was from Invitrogen.

    Control:

    Article Title: Dual targeting of lymphocyte homing and retention through α4β7 and αEβ7 inhibition in inflammatory bowel disease
    Article Snippet: Anti-αE blocking antibody (clone number M290) was from BioXcell and anti-E-cadherin antibody (clone number ECCD-2) was from Invitrogen.

    Membrane:

    Article Title: Dual targeting of lymphocyte homing and retention through α4β7 and αEβ7 inhibition in inflammatory bowel disease
    Article Snippet: Anti-αE blocking antibody (clone number M290) was from BioXcell and anti-E-cadherin antibody (clone number ECCD-2) was from Invitrogen.

    Migration:

    Article Title: Dual targeting of lymphocyte homing and retention through α4β7 and αEβ7 inhibition in inflammatory bowel disease
    Article Snippet: Anti-αE blocking antibody (clone number M290) was from BioXcell and anti-E-cadherin antibody (clone number ECCD-2) was from Invitrogen.



    Similar Products

    anti-αe blocking antibody clone number m290  (Bio X Cell)
    90
    Bio X Cell anti-αe blocking antibody clone number m290
    αEβ7 <t>or</t> <t>E-cadherin</t> blockade inhibits T cell interactions with intestinal epithelium Adoptive transfer of tdTomato + OT-1 cells into WT or E-cadherin-CFP recipients was followed by oral challenge with cholera toxin:OVA the following day. Three days after immunization, recipient mice were treated with isotype or <t>anti-αE</t> or anti-E-cadherin antibodies before analysis. (A) OT-1 cell number in the small intestine lamina propria. Bar graph shows means ± SEM of six to eight mice combined from two independent experiments. (B) Confocal microscopy image of intestinal tissue sections stained with anti-E-cadherin (green) and anti-laminin (blue). White arrows point to tdTomato (red) OT-1 T cells in close proximity to E-cadherin on basolateral epithelium. Scale bar: 100 μm. Data are representative of three independent experiments. (C) Representative movie of tdTomato (red) OT-1 T cells interacting with intestinal epithelium (green) in control or anti-αE-treated mice. The basement membrane is indicated by a dashed white line. Scale bar: 20 μm. (D and E) Maximal track speed (D) and track length (E) were quantified for individual cells. Bar graph shows means ± SEM, and each dot represents an individual cell migration event. Data are representative of five independent experiments. One-way ANOVA with Dunnett’s post-test or unpaired Student‘s t test was used to calculate statistical significance. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.
    Anti αe Blocking Antibody Clone Number M290, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti-%CE%B1e+blocking+antibody+clone+number+m290/anti+cd103+m290/pmc08385326-341-0-8
    Average 90 stars, based on 1 article reviews
    anti-αe blocking antibody clone number m290 - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    αEβ7 or E-cadherin blockade inhibits T cell interactions with intestinal epithelium Adoptive transfer of tdTomato + OT-1 cells into WT or E-cadherin-CFP recipients was followed by oral challenge with cholera toxin:OVA the following day. Three days after immunization, recipient mice were treated with isotype or anti-αE or anti-E-cadherin antibodies before analysis. (A) OT-1 cell number in the small intestine lamina propria. Bar graph shows means ± SEM of six to eight mice combined from two independent experiments. (B) Confocal microscopy image of intestinal tissue sections stained with anti-E-cadherin (green) and anti-laminin (blue). White arrows point to tdTomato (red) OT-1 T cells in close proximity to E-cadherin on basolateral epithelium. Scale bar: 100 μm. Data are representative of three independent experiments. (C) Representative movie of tdTomato (red) OT-1 T cells interacting with intestinal epithelium (green) in control or anti-αE-treated mice. The basement membrane is indicated by a dashed white line. Scale bar: 20 μm. (D and E) Maximal track speed (D) and track length (E) were quantified for individual cells. Bar graph shows means ± SEM, and each dot represents an individual cell migration event. Data are representative of five independent experiments. One-way ANOVA with Dunnett’s post-test or unpaired Student‘s t test was used to calculate statistical significance. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

    Journal: Cell Reports Medicine

    Article Title: Dual targeting of lymphocyte homing and retention through α4β7 and αEβ7 inhibition in inflammatory bowel disease

    doi: 10.1016/j.xcrm.2021.100381

    Figure Lengend Snippet: αEβ7 or E-cadherin blockade inhibits T cell interactions with intestinal epithelium Adoptive transfer of tdTomato + OT-1 cells into WT or E-cadherin-CFP recipients was followed by oral challenge with cholera toxin:OVA the following day. Three days after immunization, recipient mice were treated with isotype or anti-αE or anti-E-cadherin antibodies before analysis. (A) OT-1 cell number in the small intestine lamina propria. Bar graph shows means ± SEM of six to eight mice combined from two independent experiments. (B) Confocal microscopy image of intestinal tissue sections stained with anti-E-cadherin (green) and anti-laminin (blue). White arrows point to tdTomato (red) OT-1 T cells in close proximity to E-cadherin on basolateral epithelium. Scale bar: 100 μm. Data are representative of three independent experiments. (C) Representative movie of tdTomato (red) OT-1 T cells interacting with intestinal epithelium (green) in control or anti-αE-treated mice. The basement membrane is indicated by a dashed white line. Scale bar: 20 μm. (D and E) Maximal track speed (D) and track length (E) were quantified for individual cells. Bar graph shows means ± SEM, and each dot represents an individual cell migration event. Data are representative of five independent experiments. One-way ANOVA with Dunnett’s post-test or unpaired Student‘s t test was used to calculate statistical significance. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

    Article Snippet: Anti-αE blocking antibody (clone number M290) was from BioXcell and anti-E-cadherin antibody (clone number ECCD-2) was from Invitrogen.

    Techniques: Adoptive Transfer Assay, Confocal Microscopy, Staining, Control, Membrane, Migration